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KMID : 0355220120370020081
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Journal of Korean Academy Oral Medicine
2012 Volume.37 No. 2 p.81 ~ p.91
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The Effect of Repeated Restraint Stress on Clusterin Change of the Rat Salivary Glands
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Lee Ko-Woon
Kang Soo-Kyung
Chun Yang-Hyun
Hong Jung-Pyo
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Abstract
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It has been known that saliva may affect the most of oral diseases. On the contrary, several systemic conditions may affect salivary flow and cause oral dryness and psychosocial stress especially may a crucial role in the etiology of hyposalivation and oral dryness. Many studies have focused on macroscopic effects of the stress on the salivary glands by autonomic respose, but on the other hand it has hardly been reported on cellular microscopic effects of the stress on the salivary glands. Therefore, this study was performed to examine clusterin, a antiapoptotic and cytoprotective protein, in the parotid glands under restraint stress condition. For this study, 10 rats were divided into 3 groups; 1) 2 rats of group I were selected as a normal control. 2) 2 rats of group II, as a experimental control were placed in the restraint cone for 2 hours 3) 6 rats of group III were placed in the restraint cone for 2 hours once a day. The rats were sacrificed immediately(group II, as a experimental control), 24, 48, and 72 hours after application of the stress and the parotid glands were excised. Western blotting and immunohistochemistry were performed. The finding were as follows: 1. In parotid glands, clusterin was mildly increased and clearly expressed in the ductal cell under restraint stress immediately after application of the stress. 2. In parotid glands, clusterin was significantly decreased and slightly stained in the ductal cell under restraint stress 24 and 48 hours after experiment. 3. In parotid glands, clusterin was prominently increased again and densely stained in the ductal cell under restraint stress 72 hours after experiment.
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KEYWORD
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Restraint stress, Salivary glands, Clusterin
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